l l1 mix (New England Biolabs)
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L L1 Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 971 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l1+mix/T4+RNA+Ligase+Reaction+Buffer/pm37403796-137-65-89
Average 97 stars, based on 971 article reviews
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Multiplex Assay:Article Title: Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation Article Snippet: .. RNA was decapped by adding 10 μl Decapping Master Mix [3.25 μl 0.05% Tween, 1.5 μl T4 RNA Ligase Buffer (NEB), 4 μl PEG 8000 (50%), 0.25 μl SUPERase In RNase Inhibitor (Thermo) and 1 μl RppH (NEB)] and incubating at 37°C for ≥1 h. An RNA adapter was ligated to the decapped, monophosphorylated 5′ ends by adding 10 μl Incubation:Article Title: Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation Article Snippet: .. RNA was decapped by adding 10 μl Decapping Master Mix [3.25 μl 0.05% Tween, 1.5 μl T4 RNA Ligase Buffer (NEB), 4 μl PEG 8000 (50%), 0.25 μl SUPERase In RNase Inhibitor (Thermo) and 1 μl RppH (NEB)] and incubating at 37°C for ≥1 h. An RNA adapter was ligated to the decapped, monophosphorylated 5′ ends by adding 10 μl Ligation:Article Title: Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation Article Snippet: .. RNA was decapped by adding 10 μl Decapping Master Mix [3.25 μl 0.05% Tween, 1.5 μl T4 RNA Ligase Buffer (NEB), 4 μl PEG 8000 (50%), 0.25 μl SUPERase In RNase Inhibitor (Thermo) and 1 μl RppH (NEB)] and incubating at 37°C for ≥1 h. An RNA adapter was ligated to the decapped, monophosphorylated 5′ ends by adding 10 μl Isolation:Article Title: Combining TSS-MPRA and sensitive TSS profile dissimilarity scoring to study the sequence determinants of transcription initiation Article Snippet: .. RNA was decapped by adding 10 μl Decapping Master Mix [3.25 μl 0.05% Tween, 1.5 μl T4 RNA Ligase Buffer (NEB), 4 μl PEG 8000 (50%), 0.25 μl SUPERase In RNase Inhibitor (Thermo) and 1 μl RppH (NEB)] and incubating at 37°C for ≥1 h. An RNA adapter was ligated to the decapped, monophosphorylated 5′ ends by adding 10 μl |
